B Cell-based Flow Cytometry Assay

A flow cytometry assay for enumerating T and B cells is a powerful technique used to quantify and analyze these crucial subsets of lymphocytes within a mixed cell population. This assay involves staining the cells with fluorescently-labeled antibodies that are specific to surface markers unique to T cells, such as CD3, and B cells, such as CD19. By running the stained cells through a flow cytometer, researchers can detect and measure the fluorescence intensity, allowing for the precise enumeration and characterization of T and B cell populations based on their unique antigen markers. This method is essential in both clinical and research settings, providing valuable data on immune status, monitoring immune responses, and investigating disorders involving T or B cells, such as immunodeficiencies and leukemias. Through flow cytometry, scientists gain vital insights into immune system function and health.